Coding

Part:BBa_K2950004

Designed by: Shuxin Xiao   Group: iGEM19_BNDS_China   (2019-10-14)


ferredoxinTOL reductase (TodA)

ferredoxinTOL reductase (TodA) is involved in the toluene dioxygenase pathway, the pathway we utilized to produce our aim product urushiol, and is identified in Pseudomonas putida. TodA is responsible for the transfer of two electrons from nicotinamide adenine dinucleotide (NADH) to Rieske-type ferredoxinTOL (TodB). TodA is 44 kDa, and is characterized via SDS-PAGE electrophoresis.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1173
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 226
    Illegal AgeI site found at 319
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None